Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIVKATDQSFSAET--SEGVVLADFWAPWCGPCKMIAPVLEELDQEMGDKLKIVKIDVDENQETAGKYGVMSIPTLLVLKDGEVVETSVGFKPKEALQELVNKHL 2FCH Chain:A ((4-107)) --IIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRSIPTLLLFKNGEVAATKVGALSKGQLKEFLDANL

General information: TITO was launched using: RESULT: Template: 2FCH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 438 7814 17.84 76.60 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : 17.84 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.635

(partial model without unconserved sides chains): PDB file : Tito_2FCH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FCH-query.scw PDB file : Tito_Scwrl_2FCH.pdb: