Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRADFIQFGAMIHGVGGTTDGWRHPDVDPSASTNIEFYMKKAQTAEKGLFSFIFIADGLFISEKSIPHFLNRFEPITILSALASVTKNIGLVGTF-STSFTEPFTISRQLMSLDHISGGRAGWNLVTSPQEGAARNHSKSNL-PEHTERYEIAQEHLDVVRGLWNSWEHDAFIHNKKTGQFFDQAKLHRLNHKGKYFQVEGPL---------NIGRSKQ-GEPVVFQAGSS--ETGRQFAAKNADAIFTHSNSLEETKAFYADVKSRAADEGRDPSSVRIFPGISPIVADTEEEAEKKYREF-AELIPIENAVTYLARFFDDYDLSVYPLDEPFPDIGDVGKNAFQSTTDRIKREAKARNLTLREVAQEMAFPRTLFIGTPERVASLIETWFNAE-AADGFIVGSD-I-------PGTLDAFVEKVIPILQERGLYRQDYRGGTLRENLGLGIPQHQSVLHSSHH 4UWM Chain:A ((2-376)) -----AMETGLIFHPY--MRP----GR---SARQTFDWGIKSAVQADSVGIDSMMISEHASQIWE------NIPNPELLIAAAALQTKNIKFAPMAHLLPHQHPAKLATMIGWLSQILEGRYFLGIGAGAYPQASYMHGIRNAG--TKNLNDMVRESLFIMEKIWKR---------------------EPFFHEGKYWDAGYPEELEDEQHKLADFSPWGGKAPEIAVTGFSYNSPSMRLAGERNFKPVSIFSGLDALKRHWEVYSEAAIEAGHTPDRSRHAVSHTVFCADTDKEAKRLVMEGPIGYCFER-YLIPIWRRFG-MMD-GY------AKD---------------AGI-DPVDADLEFL-----VDNVFLVGSPDTVTEKINALFEATGGWGTLQVEAHDYYDDPAPWFQSLELISKEVAPKILLP--------------------------------

General information: TITO was launched using: RESULT: Template: 4UWM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1729 -5237 -3.03 -15.54 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -3.03 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_4UWM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UWM-query.scw PDB file : Tito_Scwrl_4UWM.pdb: