Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILFAVSECTPFVKSGGLADVAGALPKALARLGNEVAVMLPKYSQIPE--PWKKRMKKQAECTVAVGWRQQ-YCGIEHMAENDVNYYFIDNEYYFNRDSLYGH----Y-DDGERFAFFSRAVLEAAKVVN------VQADIVHTHDWHTAMVNYLLKEEYRKHPFYERMKSVLTIHNLQFQGIFPPDVTHDLLGLEMDHFH------Y--E---RLECNGFVNFMKAGIIAADHVTTVSPTYRNEIMTPYYGEQLEQVLQYREDDVTGILNGIDDTFYQPKSDPYIEAQYDSGDLACKLENKTKLQQRMGLPEKNDIPLISMVTRLTKQKGLDLVRRIMHELLEEQDIQLVVLGTGEREFEDYFRYAEFAFHEKCRA--YIGFDEPLAHQIYAGSDMFLMPSKFEPCGLGQLIALQYGAIPIVRETGGLYDTVR---------AYQEEEGTGNGFTFSAFNAHDLKFTIERALSFYCQ-QDVWKSIVKTAMNADYSWGKSAKEYQRIFEQVTRSGRDVLE 4HLN Chain:A ((125-632)) RSIVFVTGEAAP--------DVCGSLPIALAARGHRVMVVMPRYLNGTSDKNYAKALYTGKHIKIPC-FGGSHEVTFFHEYRDNVDWVFVDHPSYHRP------NFGAFGDNQFRYTLLCYAACEAPLILELGGYIYGQSCMFVVNDWHASLVPVLLAAKYRPYGVYRDSRSTLVIHNLAHQGVEPASTYP-DLGLPPEWYGALEWVFPEWARRHALDKGEAVNFLKGAVVTADRIVTVSQGYSWEVTTAEGGQGLNELLSSRKSVLNGIVNGIDINDWNPTTDKCLPHHYSVDDLSGKAKCKAELQRELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPDLMR-EDVQFVMLGSGDPVFEGWMRSTES--SYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGGLRDTVETFNPFGAKGEE------GTGWAFSPLTVEKMLWALRTAISTFREHKPSWEGLMKRGMTKDHTWDHAAEQYEQIFEWAFVDQPYV--

General information: TITO was launched using: RESULT: Template: 4HLN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2463 21510 8.73 47.07 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 8.73 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_4HLN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HLN-query.scw PDB file : Tito_Scwrl_4HLN.pdb: