Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAKFEVGSVYTGKVTGLQAYGAFVALDE---ETQGLVHISEVTH-GFVKDINEHLSVGDEVQVKVLAVDEEKGKISLSIRATQAAPEKKESKPRKPKAAQVSEEASTPQGFNTLKDKLEEWIEMSNRKDLIKK 2EQS Chain:A ((260-346)) --EEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTG--TKTSLSMKDVDQETGEDL-------------------------------------------

General information: TITO was launched using: RESULT: Template: 2EQS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 366 -2793 -7.63 -33.65 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -7.63 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.678

(partial model without unconserved sides chains): PDB file : Tito_2EQS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EQS-query.scw PDB file : Tito_Scwrl_2EQS.pdb: