Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MLAILGFVMMIVFMYLIMSNRLSA------------LIALIVVPIVFA-----LISGF--------GKDLGEMMIQGV-------TDLAPTGIMLLFA-----ILYFGI-------MIDSGLFDPLI---AKILSFVKGDPLK------------IAVGTAVLTMTISLDGDGTTTY-----------MITI------------AAMLPLYKRLGMNRLVLAGIAMLGSGVMNIIP------------WGGPTARVLASLKLDT---------SEVFTP-LIPAMIAGI------------LWVIAVAYIL---GKKERKRLGVISIDHAPSSDPEAAPLKRPAL--------QWF-----NLLLT------VALM---AALITSLLPLP----------------------VLFMTAFAVALMVNYPNVKEQQKRISAHAGNALNVVSMVFAAGIFTGIL----SGTKMVDAMAHSLVSLIPDAMGP-------HLPLITAIVSMPFTFFMSNDAFYFGVLPIIAEAASA--YGID----AAEIGRASLLGQPVHLLSPLV-PSTYLLVGMAGVSFGDHQKFTIKWAVGTTIVMTIAALLIGIISF------- 2QYU Chain:A ((8-627)) ATSSPSSPADWAKKLTDAVLRQKAGETLTAADRDFSNADFRNITFSKILPPSFMERDGDIIKGFNFSNSKFTYSDISHLHFDECRFTYSTLSDVVCSNTKFSNSDMNEVFLQYSITTQQQPSFIDTTLKNTLIRHKANLSGVILNEPDNSSPPSVSGGGNFIRLGDIWLQMPLLWTENAVDGFLNHEHNNGKSILMTIDSLPDKYSQEKVQAMEDLVKSLRGGRLTEACIRPVESSLVSVLAHPPYTQSALISEWLGPVQERFFAHQCQTYNDVPLPAPDTYYQQRILPVLLDSFDRNSAAMTTHSGLFNQVILHCMTGVDCTDGTRQKAAALYEQYLAHPAVSPHIHNGLFGNYDGSPDWTTRAADNFLLLSSQDSDTAMMLSTDTLLTMLNPTPDTAWDNFYLLRAGENVSTAQISPVELFRHDFPVFLAAFNQQATQRRFGELIDIILSTEEHGELNQQFLAATNQKHSTVKLIDDASVSRLATIFDPLLPEGKLSPAHYQHILSAYHLTDATPQKQAETLFCLSTAFARYSSSAIFGTEHDSPPALRGYAEALMQKAWELSPAIFPSSEQFTEWSDRFHGLHGAFTCTSVVADSMQRHARKYFPSVLSSILPLAWA

General information: TITO was launched using: RESULT: Template: 2QYU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2124 -140615 -66.20 -330.08 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -66.20 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.179

(partial model without unconserved sides chains): PDB file : Tito_2QYU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QYU-query.scw PDB file : Tito_Scwrl_2QYU.pdb: