Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLFIQNDQQRQWMEKIGRIADEFQQTAAEDDEQGRFPAEKIQKLRDAGYTALTLPASHGGGGIS-VYDMLLFQERLARGDAPTALSIGWHLSVIGELGEGNSW--DEDVFAFVAKEVQNGAVINRAATEAKTGSPTRGGRPGTHAVKKDGK--WAVNGRKTFTTMSQALDYFLVTAWIEDKQTTGVF---LIHKDDPGLSIEETWDMMAMRATGSHDLVLNEVMLDENKLVELLQGPRGAKPNGWL-------LHIPAIYLGVAQAARDYAVQFASEYSPNSLNGPIKNVPAVQQRTGEMELE----LLNARHFLFHIAQLYDDPVRRPHLTSELGAAKHIVTNAALSVVDKAMRIVGAKSLERTNPLQRYYRDVRAGLHNPPMDDAVIHKLAAEAFES 4KCF Chain:A ((40-398)) ----------RVLLDRLAAHLPRIRSTAAEHDRDGTFPTDTFDALRKDGLMGATVPAELGGLGVDRLYDVAVALLAVARADASTALAL--HMQLSRGLTLGYEWRHGDERARTLAERILRGMV----AGDAVVCSGIKDHHTAVTTLRPDGAGGWLLSGRKTLVSMAPVGTHFVINARTDGTDGPPRLASPVVTRDTPGFTVLDNWDGLGMRASGTVDIVFDDCPIPADHV--LMRDPVGARNDAVLAGQTVSSVSVLGVYVGVAQAAYDTAVAALERRPEPPQAAALTLVAEIDSRLYALRATAGSALTAADALSADLSGDMDE--RGRQMMRHFQCAKLAVNRLAPEIVSDCLSLVGGASYTAGHPLARLLRDVQAG---------------------

General information: TITO was launched using: RESULT: Template: 4KCF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1794 -161888 -90.24 -476.14 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -90.24 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_4KCF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KCF-query.scw PDB file : Tito_Scwrl_4KCF.pdb: