Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPINSFENYPMSWKPSIDKAEKPIYKALAGQLEQDILNGVLLPGTKLPPQRELADYLDLNVSTISKAFKVCELKGLLSATVGSGTFVSYDALSNAYLLEDTKPTHLIEMGAILPDHASYEPLLYQLKNMVHEEDYEKWFSYGRAGESLWQKDAAVKLIQRGGFETSVDHILFANGGQNAIAATLASLCKPGDRIGVDHHTYPGLKTVASLLSVQIVPIKSENDEMSPESLE---YACKNENIKG--------LYLIPDYHNPTTSFMSVENRRMVADIVKKYNLFVIEDASYHLL--NKNPLPALASF-APQQVIHIASLSKSLAPGLRLAYVAVPRQYKEPI---------SKALYNLNITVSPLLAELTARTIVSNQFEVLIESHREQTIRRNQLVNRYVAGYTCLGV-ETGIFRWLLLPGKMSGAEF-EELAARLGVQVYAAERFVVGNSCPERAVRVSVCAPKTLEELEQGLMILRRLLDDLT 5EFS Chain:A ((114-423)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------TSGSQQGLCKVFEMIINPGDNVLLDEPAYSGTLQSLHPLGCNIINVASDESGIVPDSLRDILSRWKPEDAKNPQKNTPKFLYTVPNGNNPTGNSLTSERKKEIYELARKYDFLIIEDDPYYFLQFNKFRVPTFLSMDVDGRVIRADSFSKIISSGLRIGFLTGPKPLIERVILHIQVSTLHPSTFN-QLMISQLLHEWGEEGFMAH-VDRVIDFYSNQKDAILAAADKWLTGLAEWHVPAAGMFLWIKVKGINDVKELIEEKAVKMGVLMLPGNAFYVDSSAPSPYLRASFSS-ASPEQMDVAFQVLAQLIKE--

General information: TITO was launched using: RESULT: Template: 5EFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1354 -153788 -113.58 -539.61 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -113.58 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_5EFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EFS-query.scw PDB file : Tito_Scwrl_5EFS.pdb: