Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIRNPFKDKYYSHDRRALNMLALRVPGLAFILMIYIASIVLQFVSGGWSILLLYAFTILIAIFALLHWHSYRWVKKRVILYFAVQGLITFALANLMTGFFILVIIGLYAFLIGQIIGMADRRRTFLILYLLLLLVINSAYHLHKGEVLHFIVIAAPIMIVIITYAATFFAQVDEKIKAQLTLERLELAHQQVEQLTLQNERQRMARDLHDTLAQGLVSLNMQLDAIHVHLAKGNTERAKEIIQQSMKRVKSTIADARSAIDDLRS-KSEEIGV-LKERITSLMDHFIESTGMACLL-DYRLHQVLDVRTAENCYYIIGECMTNAAKHAEAKTIWISI---WDDEKGRLHLTVKDNGKGFDVEKGKKKRGHYGLLGIQERVRAINGQFNIKSTKLKGTQIEITVPIQGEMQDE 3EHH Chain:A ((16-214)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NERIAELVKLEERQRMARDLVDTLGQKLSLMGLKSDLARKLIYKDPEQAAREL--------KSVQQTARTSLNEVRKIVSSMKGIRLKDELINIK-QILEAADIMFIYEEEKWPENISLLNENILSMCLKEAVTNVVKHSQAKTCRVDIQQLWKE----VVITVSDDGT-FKG--------H-GLLGMRERLEFANGSLHIDTE--NGTKLTMAIP--------

General information: TITO was launched using: RESULT: Template: 3EHH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 670 -68921 -102.87 -372.54 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -102.87 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_3EHH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EHH-query.scw PDB file : Tito_Scwrl_3EHH.pdb: