Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGFSHFNEQGRAQMVDISEKSSTVRTAAAVSSVHMKNEVYEKIQSHDIGKGDVLAVAQVAGIMAAKQTSNIIPMCHPLSLSGVDISFDWKIKEAEVILHIKAQVKTKGSTGVEMEALTSASVCALTVYDMCKALDKGMVIGPTFLLEKTGGKNGDFKRELSEYNLEDQK 3JQJ Chain:A ((9-155)) ----------GRPRMVDVTEKPETFRTATAEAFVELTEEALSALEKGGVGKGDPLVVAQLAGILAAKKTADLIPLCHPLPLTGVEVRVE--LLKAEKRVRIEATVKTKAETGVEMEAMTACAVAALTVYDMLKAASKGLVISQVRLLHKAGGKSGEWRR-----------

General information: TITO was launched using: RESULT: Template: 3JQJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 761 -78807 -103.56 -536.10 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -103.56 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_3JQJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JQJ-query.scw PDB file : Tito_Scwrl_3JQJ.pdb: