Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRMKVNQMSLLEHIAELRKRLLIVALAFVVFFIAGFFLAKPIIVYLQETDEAKQLTLNAFNLTDPLYVFMQFAFIIGIVLTSPVILYQLWAFVSPGLYEKERKVTLSYIPVSILLFLAGLSFSYYILFPFVVDFMKRISQDLNVNQVIGINEYFHFLLQLTIPFGLLFQMPVILMFLTRLGIVT-PMFLAKIRKYAYFTLL-VIAALITPP-----ELLSHMMVTVPLLILYEISILISKAAYRKAQKSSAADRDVSSGQ 1DTS Chain:A ((138-214)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLPVILVVGVKLGCINHAMLTAQVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAEAA-------------------

General information: TITO was launched using: RESULT: Template: 1DTS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 202 -56612 -280.26 -857.76 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.50 3D Compatibility (PKB) : -280.26 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_1DTS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DTS-query.scw PDB file : Tito_Scwrl_1DTS.pdb: