Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKGRGLEILINEKQDGQWLFSVLKTALKASKPVIQDWMSHQQIKVNHESVLNNMIVKKGDRVFIDLQESEASSVIPEYGELDILFEDNHMLIINKPAGIATHPNEDGQTGTLANLIAYHYQINGETCKVRHVHRLDQDTSGAIVFAKHRLAHAILDQQLEKKTLKRTYTAIAEGKLRTKKGTINSPIGRDRSHPTRRR---VSPGGQTAVTHFKVMAS-NAKERLSLVELELETGRTHQIRVHLASLGHPLTGDSLYGGGSKL--------------LNRQALHANKVQAVHPITDELIVAEAPFPADMKNLCRTYFS 1PRZ Chain:A ((79-309)) -----------------------------------------------------------------------------PQDIPLDIVYEDEDIIVINKPRDLVVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVESLQRREITREYEAVAIGHM-TAGGTVDEPISR---HPTKRTHMAVHPMGKPAVTHYRIMEHFRVHTRL---RLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDRQALHATMLRLYHPISGIEMEWHAPIPQDMVELI-----

General information: TITO was launched using: RESULT: Template: 1PRZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1149 -89512 -77.90 -420.24 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -77.90 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_1PRZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PRZ-query.scw PDB file : Tito_Scwrl_1PRZ.pdb: