Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTWRKSYERWKQTEHLDLELKERLIELEGDEQALEDCFYKDLEFGTGGMRGEIGAGT-NRMNIYTVRKASAGFAAYISKQGEEAKKRGVV----IAYDSRHKSPEFAMEAAKTLATQGIQTYVFDE--LRPTPELSFAVRQLNAYGG-----IVVTASHNPPEYNGYKVYGDDGGQLPPKEADIVIEQVNA-----IENELTITVDEENKLKEKGLIKIIGEDIDKVYTEKLTSISVHPELSEEVDVKVVFTPLHGTANKPVRRGLEALGYK-NVTVVKEQELPDSNFSTVTSPNP---EEHAAFEYAIKLGEEQNADILIATDPDADRLGIAVKNDQGKYTVLTGNQTGALLLHYLLSEKKKQGILPDNGVVLKTIVTSEIGRAVASSFGLDTIDTLTGFKFIGEKIKEYEASGQYTFQFGYEESYG--YLIGD---FARDKDAIQAALLAVEVCAFYKKQGMSLYEALINLFNEYGFYREGLKSLTLKGKQGAEQIEAILASFRQNPPQKMAGKQVVTAEDYAVSKRTLLTESKEEAIDLPKSNVLKYFLEDGSWFCLRPSGTEPKVKFYFAVKGSSLEDSEKRLAVLSEDVMKTVDEIVESTAK 2FUV Chain:A ((43-521)) -----------------------------------------VKFGTSGHRGSAGRHSFNEPHILAIAQAIA----------EERAKNGITGPCYVGKDTHALSEPAFISVLEVLAANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPPNGGPADTNVTKVVEDRANALLAGGLQGVKRISLDAA---MASGHVKAV--DLVQPFVEGLADI-VDMAAIQKAGLTLGVDPLGGSGIEYWKRIAEH--YKLNLTLVNDQVDQTFRFMHLDKDGAIRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVT-----PAGLMNPNHYLAVAINYLFQHRPLWG---KDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGL--FDGS----FGFGGEESAGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAARFGAPSYNR----------------LQASATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASI---GGLKVMTDNG-WFAARPSGTEDAYKIY-----------------------------------

General information: TITO was launched using: RESULT: Template: 2FUV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2621 -147473 -56.27 -325.55 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -56.27 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_2FUV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FUV-query.scw PDB file : Tito_Scwrl_2FUV.pdb: