Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYDIISDIHGCYDEMTALIQKLGYTIKNGVPVHEEGRVLVFAGDLTDRGPKSIEVIRFVAGAYEKGAVRYVPGNHCNKLYRYL--KGNPVKVMHGLETTAAELEELSKDEKKSVSEQFMKLYETAPLYDILHNGELVVAHAGIRADDIGKYTRRVKDFVLYGDVTGETYPDGRPI--------RRDWAAAYN-------GKAWVVYGHTPVKEPRKVNRTINIDTGCVFGNQLTGFRFPEIETVSVPSSLPYDESRFRPI 1G5B Chain:A ((17-212)) ----VVGDLHGCYTNLMNKLDTIGFDNKK--------DLLISVGDLVDRGAENVECLELITFPW----FRAVRGNHEQMMIDGLSERGN---VNHWLLNGGGWFFNLDYD-KEILAKALAHKADELPLII-----ELV--------------SKDKKYVICHADYPFDEYEFGKPVDHQQVIWNRERISNSQNGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLT--------------------------

General information: TITO was launched using: RESULT: Template: 1G5B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 882 -100387 -113.82 -560.82 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -113.82 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_1G5B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G5B-query.scw PDB file : Tito_Scwrl_1G5B.pdb: