Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATIRIHDEANTTIENQEEVASFLDSQEVIYEQWDITRLPEHLSEKYDL-TEEEKQQILDTFETEIKDISTRRGYKAQDVISLSDSNPKLDELLENFKREHHHTDDEVRFIVSGHGIFVIQGQDGTFFDVRLNPGDLISVPENIRHYFTLQEDRKVVAVRIFVTTEGWVPIYEKDSVNQ 2HJI Chain:A ((20-156)) ---------------NAEEIQQQLNAKGVRFERWQADR---------DLGAAPTAETVIAAYQHAIDKLVAEKGYQSWDVISLRADNPQKEALREKFLNEHTHGEDEVRFFVEGAGLFCLHIGD-EVFQVLCEKNDLISVPAHTPHWFDMGSEPNFTAIRIF-----------------

General information: TITO was launched using: RESULT: Template: 2HJI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 550 -72472 -131.77 -532.88 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -131.77 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_2HJI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HJI-query.scw PDB file : Tito_Scwrl_2HJI.pdb: