Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILYTVMPQEIVFAEQNQETSAHEQIEYKGVPLLVEMKG-NEAEVIQIMSTNPM-HFLHPDISPGQKLKLNV 4QIW Chain:H ((30-63)) ----------------------------------LKKYRIRISQLPQIKASDPAVVALG--AKPGDVIEI--

General information: TITO was launched using: RESULT: Template: 4QIW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 43 -7671 -178.40 -239.72 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain H : 0.57 3D Compatibility (PKB) : -178.40 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.145

(partial model without unconserved sides chains): PDB file : Tito_4QIW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QIW-query.scw PDB file : Tito_Scwrl_4QIW.pdb: