Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKTTGHTTIKDVAECAGVSKSTVSRYINGKIDAISPEKVKNIKKAIAELNYRPSKMAQGLKIKKSKLIGFVVADITNPFSVAAFRGVEEVCDQYGYSIMVCNTDNSPEKEREMLLKLEAHSVEGLILNATGENKDVLRAFAEQQIPTILIDRKLPDLKLDTVTTDNRWITKEILQKVYSKGYTDVALFT----EPISSISPRAERAAVYQEMASVQNVNGLVRLHEIDVKDKEQLKAELRSFHKEMPEQKK--AILALNGLIMLKIISCMEELGLRIPQDIGIAGFDDTEWYKLIGPGITTIAQPSHDMGRTAMERVLKRIEGDKGAPQTIELEAKVIMRKSL 1ZVV Chain:A ((2-330)) -------NVTIYDVAREASVSMATVSRVVNGNPN-VKPSTRKKVLETIERLGYRPNAVARGLASKKTTTVGVIIPDISNIFYAELARGIEDIASMYKYNIILSNSDQNQDKQLHLLNNMLGKQVDGIIFMSGNVTEEHVEELKKSPVPVVLAASIESTNQIPSVTIDYEQAAFDAVQSLIDSGHKNIAFVSGTLEEPIN----HAKKVKGYKRALT---ESGLPVRDSYIVEGDYTYDSGIEAVEKLLEEDEKPTAIFVGTDEMALGVIHGAQDRGLNVPNDLEIIGFDNTRLSTMVRPQLTSVVQPMYDIGAVAMRLLTKYMNKETVDSSIVELPHRIEFRQS-

General information: TITO was launched using: RESULT: Template: 1ZVV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1670 -66329 -39.72 -205.35 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -39.72 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_1ZVV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZVV-query.scw PDB file : Tito_Scwrl_1ZVV.pdb: