Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLDKLLANSGYGSRKEVKAVVKAGAVMIDGKPAKDVKEHVDPDTQEVTVYGEP---VDYREFIYLMMNKPQGVLSATEDSRQQTVVDLLTPEEMRFEPFP-------AGRLDKDTEGFLLLTNDGQLAHRLLSPKKHVPKTYEVHLKSQISREDISDLETGVYIEGGYK-TKPAKAEIKTNDSGNTVIYLTITEGKYHQVKQMAKAVGNEVVYLKRLSMGRVSLDPALAPGEYRELTEEELHLLNEPQA 1VIO Chain:A ((3-233)) LRLDKFIAENVGLTRSQATKAIRQSAVKINGEIVKSGSVQI---SQEDEIYFEDELLTWIEEGQYFMLNKPQGCVCSNDD--YPTI--------YQFFDYPLAGKLHSAGRLDVDTTGLVLLTDDGQWSHRITSPKHHCEKTYLVTLADPVEENYSAACAEGILLRGEKEPTKPAKLEILDDYNVN----LTISEGRYHQVKRMFAALGNKVVGLHRWKIGDVVLDESLEEGEYRPLTQSEIEKLV----

General information: TITO was launched using: RESULT: Template: 1VIO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1051 13122 12.48 60.19 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 12.48 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_1VIO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VIO-query.scw PDB file : Tito_Scwrl_1VIO.pdb: