Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEADMLFSVTVPGSTANLGPGFDSVGMALSR-YLKLTVFESDKWSFEAETETVAGIPAGTD-NLIYQVAKRTADLY--GKEMPPVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSEADKLHLASLEE------GHPDNAGASLVGGLVIGLHEDDETQMIRVP-NADIDVVVVIPFYEVLTRDARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRAMLVPELSKVEHVAEMKGAYGTALSGAGPTILVMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIERNPLYQVKSV 1FWK Chain:A ((1-283)) -----MKVRVKAPCTSANLGVGFDVFGLCLKEPYDVIEVEAIDDKEIIIEVDD-KNIPTDPDKNVAGIVAKKMIDDFNIGK---GVKITIKKGVKAGSGLGSSAASSAGTAYAINELFKLNLDKLKLVDYASYGELASSGAKHADNVAPAIFGGFTM-VTNYEPLEVLHIPIDFKLDILIAIPNISINTKEAREILPKAVGLKDLVNNVGKACGMVYALYNKDKSLFGRYMMSDKVIEPVRGKLIPNYFKIKEEVKDK-VYGITISGSGPSIIAFPKEEFIDEVENILRDYYEN--------------------------

General information: TITO was launched using: RESULT: Template: 1FWK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1573 34854 22.16 128.14 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 22.16 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_1FWK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FWK-query.scw PDB file : Tito_Scwrl_1FWK.pdb: