Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMAKKCSVFMLCAAVSLSLAACGPKESSSAK-SSSKGSELVVWE---DKEKSNGIKDAVAAFEKEHDVKVKVVEKPYAKQIEDLRMDGPAGTGPDVLTMPGDQIGTAVTEGLLKELHVKKDVQSLYTDASIQSQMVDQKLYGLPKAVETTVLFYNKDLITEKELPKTLEEWYDYSKKT--ADGSKFGFLALFDQIYYAESVMSGYGGYIFGKAKDGSYNPSDIGINNEGAVKGAALIQKFYKDGLFPAGIIGEQGINVLESLFTEGKAAAIISGPWNVEAFSNAGINYGITKLPKLENGKNMSSFIGVKSYNVSAFSKNEELAQELAVFLANEKNSKTRYEETKEVPAVKSLANDPAIMKSEAARAVTEQSRFSEPTPNIPEMNEIWTPADSALQTVATGKADPKQALDQAAETAKGQIKAKHSGK 2ZYO Chain:A ((13-393)) --------------------------------KSEGKPDKLVVWENADDGVQLNNTKKWAGEFTKKTGIQVEVVPVALLKQQEKLTLDGPAGKGADLVTWPHDRLGEAVTKGLLQPIQVDNSVKNQFDDVAMKALTYGGKLYGLPKAIESVALIYNKKLMGQ--VPATYDELFQYAKANNKPDEQKYGVLFEANNFYYTYFLFAAKGAAVF-KEQDGTLDPNEIGLNSPEAVQGMNEVQKWFTEARLPQSLKAD----TVNGLFKSGKVAAVINGPWAIKDYQAAGINVGVAPLPKID-GKDAQTFIGVKGWYLSAYSKYPKYATELMQFLTSKEALASRFKETGEIPPQKELLNDPMIKNNPVVNGFAKQASKGVPMPSIPEMGVVWEPINNAHTFVAQGKQTPEQALNDAVKIMKEKIQ------

General information: TITO was launched using: RESULT: Template: 2ZYO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2093 118898 56.81 317.06 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 56.81 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_2ZYO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZYO-query.scw PDB file : Tito_Scwrl_2ZYO.pdb: