Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHGYRTIEFLGLTFNLTNILMITVASVIVLLIAILTTRTLSIRPGKAQNFMEWIVDFVRNIIGSTMDLKTGANFLALGVTLLMYIFVSNMLGL-PFSITIGHELWWKSPTADPAITLTLAVMVVALTHYYGVKMKGLKEYSKDYL-------RPVPFMLPMKIIEE----FANTLTLGLRLYGNIFAGEILLGLLAGLATSHYSQSVALGLVG-TIGAILPMLAWQAFSLFIGAIQAFIFTMLTMVYMSHKISHDH 5ARH Chain:W ((14-225)) -------------------ILGLPLVTLIVLFPSLLFPTSNRLVSNRFVTLQQWMLQLVSKQMMSIHNSK-GQTWTLMLMSLILFIGSTNLLGLLPHSFT---------PTTQLSMNLGMAIPL-----WAGAVITGFRNKTKASLAHFLPQGTPTP-LIPMLVIIETISLFIQPMALAVRLTANITAGHLLIHLIGGATLALMSISTTTALITFTILILLTILEFA-----VAMIQAYVFTLLVSLYL-----HDN

General information: TITO was launched using: RESULT: Template: 5ARH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain W - contact count / total energy / energy per contact / energy per residue : 700 -102091 -145.84 -513.02 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain W : 0.73 3D Compatibility (PKB) : -145.84 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.304

(partial model without unconserved sides chains): PDB file : Tito_5ARH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ARH-query.scw PDB file : Tito_Scwrl_5ARH.pdb: