Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKEFKAESKRLLDMMINSIYTQKEIFLRELISNSSDAIDKIYYKALTDDALTFDKDSYYIKVAADKDARTLTISDTGIGMTKDELEQHLGTIAKSGSLAFKKENELKDGHDI--IGQFGVGFYAAFMVADVVTVISKALGSEEAYKWESAGADGYTIEPCE-KDSVGTDIILKIKENTEDDSYDEFLEEYRLKAIIKKYSDFIRYPIKMDTTINKPKEGSENEFEEVQEEQTVNSMVPIWRKNKSELTDEDYEKFYAEKHYGFDKPLTHVHISVDGAVRYNAILFVPENMPFDYYSKEYEKGLELYSNGVLIMNKCADLLPDHFSFVKGMVDSEDLSLNISREMLQHDRQLKLIAKNISKKIKSELQSLLKKDREKYETFYKSFGRQLKFGVYNDFGANKDQLKDLLLFYSSKE-KKLVSLDEYVSRMPEEQKFIYYATGDSYDRIEKLPQTEMVADKGYEILYFTEDIDEFAIKMLASYQEKEFKSVSSGDLGIDTDEDQKQSEEEESKYKDLFEEMKNILSDKVKNVRISKRLKSHPVCLAADGEV--TIEMEKILNAMPDSQNVKAEKVLEINPNHEVFQTLQ----DAFEQDKEKLSLYTNLLYNQALLIEGLPIQDPVEFTNNICKVMV 2CG9 Chain:A ((7-672)) ----EFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEA--LSAGADVSMIGQFGVGFYSLFLVADRVQVISKS-NDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRL--FLKDDQL-EYLEEKRIKEVIKRHSEFVAYPIQLVVTKEVEKE------EEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPF--------NNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEI-AEDSEQFEKFYSAFSKNIKLGVHED-TQNRAALAKLLRYNSTKSVDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRT-GQFGWSANMERIMKAQA--------KTFEISPKSPIIKELKKRVDEGGAQDKTVKDL-TKLLYETALLTSGFSLDEPTSFASRINRLI-

General information: TITO was launched using: RESULT: Template: 2CG9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2737 53839 19.67 91.41 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 19.67 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_2CG9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CG9-query.scw PDB file : Tito_Scwrl_2CG9.pdb: