Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGELANYKRLEKVGEGTYGVVYKALDLRPGQGQRVVALKKIRLESEDEGVPSTAIREISLLKELKDDNIVRLYDIVHSDAHKLYLVFEFLDLDLKRYME-----GIPKDQPLGADIVKKFMMQLCKGIAYCHSHRILHRDLKPQNLLINKDGNLKLGDFGLARAFGVPLRAYTHEIVTLWYRAPEVLLGGKQYSTGVDTWSIGCIFAEMCNRKPIFSGDSEIDQIFKIFRVLGTPNEAIWPDIVYLPDFKPSFPQWRRKDLSQVVPSLDPRGIDLLDKLLAYDPINRISARRAAIHPYFQES 4EOK Chain:A ((3-291)) ---SMENFQKVEKIGEGTYGVVYKARNKLTGE---VVALKKIRL----EGVPSTAIREISLLKELNHPNIVKLLDVIHTE-NKLYLVFEFLSMDLKDFMDASALTGIP------LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQD-

General information: TITO was launched using: RESULT: Template: 4EOK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -181780 for 2221 contacts (-81.8/contact) + 2D Compatibility (PS) -29497 + (NN) -10078 + (LL) 248 1D Compatibility (HY) -38800 + (ID) 9200 Total energy: -269107.0 ( -121.16 by residue) QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_4EOK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EOK-query.scw PDB file : Tito_Scwrl_4EOK.pdb: