Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTIIACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRCFDTKAQI-CSPQEFKDLETAFNQMSSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLNTISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENRQVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIIDQGYGIKDEDLASIFNRLYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKGSKFSLVLKLQK 4BIW Chain:A ((52-296)) -------------------------------------------------------------------------------------LEAGPQEFLAAGASFNQVVTALERMMTS-------QQRLLSDISHELRTPLTRLQLGT-ALLRRRSGESK---ELERIETEAQRLDSMINDLLVMSRNQQKNAL---VSETIKANQLWSEVLDNAAFEAEQMGKSLTVNFPPGPWPLYGNPNALESALENIVRNALRYSH--TKIEVGFAVDKDGITITVDDDGPGVSPEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIWLPLYK

General information: TITO was launched using: RESULT: Template: 4BIW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -83271 for 1791 contacts (-46.5/contact) + 2D Compatibility (PS) -25125 + (NN) -1102 + (LL) 8664 1D Compatibility (HY) -9600 + (ID) 3200 Total energy: -113634.0 ( -63.45 by residue) QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_4BIW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BIW-query.scw PDB file : Tito_Scwrl_4BIW.pdb: