Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNLNEKQLITEIVGLARSQGLTVHSENAQLNETGMDFQVVFAKDDTGMPWVLRKPRRSDVVERASAEGITLAFLRANLTADVPDWRIHTPELIAYPMLKGTPAAGIDLEQKQYVWNMDHQPPSDDFVRTLADILAELHGTDQISAGQSGIEVIRPEDFRQMTADSMVDVKNKLGVSTTLWERWQKWVDD--------DAYWPGFSSLIHGDLHPPHILID-QNGRVTGLLDWTEAKVADPAKDFV---LYQTIFGEKETARLLEYYDQAGGRIWAKMQEHISEMQAAYPVEIAKLALQTQQEEHINMALEALGVTSD 4DE4 Chain:A ((142-273)) --------------------------------------------------------------------------------------------------------------------------------KDLARFLSELHSIN-ISGFKSNLVL----DFREKINEDNKKIKKLL--SRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHY---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4DE4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 327 -47316 -144.70 -394.30 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -144.70 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_4DE4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DE4-query.scw PDB file : Tito_Scwrl_4DE4.pdb: