Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVLWVAAVIALACLNVIQFIMKKKRDGNLAYTADQLSYMLSRDSAGQILLPTDDHALKDLLVNINLIVENRQQISAQFAKTEQSMKRMLTNMSHDLKTPLTVILGYIEAIQSDPNMPD-EERERLLGKLRQKTNELIQMINSFFDLAKLESEDKEIPITKVHMNDICKR--NILHYYDAVQSKGFQAAIDIPDTPVYAQANEEALDRILQNLLSNAIQYG---AAGKLIGLTLSYDERNIAITVWDRGKGISETDQQRVFERLYTLEESRNKAFQGSGLGLTITKRLTEKMGGIISVQSKPYERTAFTITLKRMTY 4JAS Chain:A ((23-247)) ----------------------------------------------------------------------------------------FIANISHELRTPLTVIKGYAEMIYNSLGELDLSTLKEFLETIIEQSNHLENLLNELLDFSRLERKSLQINREKVDLCDLVESAVNAIKEFASSHNVNVLFESNVP-CPVEAYIDPTRIRQVLLNLLNNGVKYSKKDAPDKYVKVILDEKDGGVLIIVEDNGIGIPDHAKDRIFEQFYRVDSSLTYEVPGTGLGLAITKEIVELHGGRIWVESEVGKGSRFFVWIPK---

General information: TITO was launched using: RESULT: Template: 4JAS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 894 -100922 -112.89 -460.83 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -112.89 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_4JAS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JAS-query.scw PDB file : Tito_Scwrl_4JAS.pdb: