Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEMFDLEFMRRAFLAGGMIAVMAPILGVYLV--LRRQ--ALMADTLSHISLSGVAIGFFLSTN---ITAASIVVVTIGAIGIEYMRRAYRTYSEVSIAILMAAGLSFAMFLISLS-------KG-TANMSIDQYLFGSLVTVNQQQVYIISIITLLILLYFIVLRRPLYLLTFDEATAKTSGINTNVLSLSFSIVTGLAISVIIPIIGVLLVSALLVLPAAFAIRIAKGFNMVFITAILISLFSVFTGLTSSY---QLGTPPGPSITLLLIVLLLIGFAVQGVWTFIKKEAQRKKRSR 2NQ2 Chain:A ((63-327)) ---------LPRILTALCVGAGLALSGVVLQGIFRNPLVNPHIIGVTSGSAFGGTLAIFFGFSLYGLFTSTILFGFGTLALVFLFSFK------SLLMLILI-GMILSGLFSALVSLLQYISDTEEKLPSIVFWLMGSFATSNWEKLLFFFVPFLLCSSILLSLSWRLNLLSLDEKEAKALGVKMAPLRWLVIFLSGSLVACQVAISGSIGWVG-LIIPHLSRMLVGANHQSLLPCTMLVGATYMLLVDNVARSLSDAEIPISILTALIGAPLFGVLV--------------------

General information: TITO was launched using: RESULT: Template: 2NQ2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1175 -247595 -210.72 -1018.91 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -210.72 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_2NQ2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NQ2-query.scw PDB file : Tito_Scwrl_2NQ2.pdb: