Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNFILNENKQLSLAIEDENIENFYIDGTDLVRKIIRRSGSGVTSRVPVLSTQDLENKNLHELYDESWLRMKNRPNTELTTESINIADLFSGCGGLSLGVWEACRALGINPRFSFACDLNEAALSVYEKNFSPDFSLNESIEKHINGELGAPLTVEEQRIKDKVKKIDFILAGPPCQGHSDLNNHTRRKDPR-NALLMRVSRVIELFQPSSVLVENVPGIIHDKSGSFKEFKNHLKTQGYYFDEIVLNAEKLGVSQARRRYFIFASKTPVS-SLNQINEFYSTNSRP--ISWAISDLVENVGDDIFNTASEHSLENKRRIEYLFENNLFELPNSERPDCHRLKPHSYKSVYGRMYWDRPAPTITRGFGSTGQG-----RFVH---SLLKRTITPHEAARIQFFPDFFNF-GDLRRRQYQDVIGNAVPSKLSYLLALHQLR 4H0N Chain:A ((6-323)) -------------------------------------------------------------------------------------ILELYSGIGGMHCAWKES----GLDGEIVAAVDINTVANSVYKHNFPETNLLNRNIQQ-----------LTPQVIKK--WNVDTILMSPPCQPFTRNGKYLDDNDPRTNSFLYLIGILDQLDNVDYILMENVKGF--ENSTVRNLFIDKLKECNFIYQEFLLCPSTVGVPNSRLRYYCTARRNNLTWPFKRRDEIITRLPKDFGVPHSLESIIEEDVDEKF-LVPEKMLRCAKVFDICYK-------TSKRSCCFTKAYTHYADGTGSIFTDKPREVVQKCYAAAAQNEIGGEKFVELFKELKLRYFTPKEVLMIMCFPKSYNLPTNISMKQCYRLLGNSVNVKV----------

General information: TITO was launched using: RESULT: Template: 4H0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1444 -145427 -100.71 -476.81 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -100.71 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_4H0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H0N-query.scw PDB file : Tito_Scwrl_4H0N.pdb: