Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISYKCPNCGSDMAFDSETGSLSCSSCGRQDNIESLPKENIAARFSDDEAKEYQCKNCGAVLITEAETTATTCSFCGGAAILADRLSGHLAPAKVIPFTISKQEAEQAFRKWCKKGLLTPRGFMSADRIKSITGMYIPFWMFDLNSEVQVRANCTRVHQYEEGD-YICTETE--HFEAFRDINLDYLKIPVDASEKMKDELMDKLEPYSYEELKDFQTAYLAGYIAEKYNYTDEELFPRAKEKISSYIDSYIHSTFSGYTSVNVRDKHIHTKNVNSFYVLLPVWMVSYDYERAEHIFAMNGQTGKVVGKPPISRGKVAAWFSGIAGGTFLALKLVSLMMGGGF 2XU0 Chain:A ((345-443)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------KYCSGDGHDCTQTNLAHNQIFVDLDCPRCQ---DQCIKYNEWIVKKLEEFYKQNLK--YSMEIQKWKKTKNNYYDKEFYENLDKKSYSTIDKFLNLLNNG--------KHCH-------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2XU0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 172 -6592 -38.33 -68.67 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -38.33 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.205

(partial model without unconserved sides chains): PDB file : Tito_2XU0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XU0-query.scw PDB file : Tito_Scwrl_2XU0.pdb: