Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFHISTLDQIKIAYIGGGSQGWARSLMS---DLSIDERMSGTVALYDLDFEAAQKNEVIGNHSGNGRWRYEAVSTLKKALSAADIVIISILPGSLDDMEVDVHLPERCGIYQSVG-DTVGPGGIIRGLRAVPIFAEIARAIRDYAPESWVINYTNPMSVCTRVLYKVFPGIKAIGCCHEVFGTQKLLAEMVTERLGIEVPRREDIRVNVLGINHFTWITKASYRHIDLL-PIFREFSAHYGESGYELEGECWRDSVFCSAHRVAFDLFETYGAIPAAGDRHLAEFLPGPYLKQPEVWKFHLTPISFRKQDRAEKRQETERLIVQQRGVAE-----KASGEEGVNIIAALLGLGELVTN------VNMPNQGQVLNLPIQAIVETNAFITRNRVQPILSGALPKGVEM-LAARHISN--QEAVADAGLTKDTGLAFQAFLNDPLVQIDRSDAEQLFNDMLQCIMQS 1UP7 Chain:A ((2-409)) --------HMRIAVIGGGS-SYTPELVKGLLDISEDVRID-EVIFYDIDEEKQKIVVDFVKRLVKDRFKVLISDTFEGAVVDAKYVIFQFRPGGLKGRENDEGIPLKYGL---IGQETTGVGGFSAALRAFPIVEEYVDTVRK-TSNATIVNFTNPSGHITEFVRNYLEYEKFIGLCNVPINFIREIAEMFSARL-------EDVFLKYYGLNHLSFIEKVFVKGEDVTEKVFENLKLK---IPDEDFPTWFYDSV---------------------------RLIVNPYLRYYLMEKKMFKKISTHELRAREVMKIEKELFEKYRTAVEIPEELTKRGGSMYSTAAAHL-IRDLETDEGKIHIVNTRNNGSIENLPDDYVLEIPCYVRSGRVHTLSQG---KGDHFALSFIHAVKMYERLTIEAYLKRSKKLALKALLSHPLGP-DVEDAKDLLEEILEA----

General information: TITO was launched using: RESULT: Template: 1UP7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2078 -211977 -102.01 -549.16 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -102.01 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_1UP7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UP7-query.scw PDB file : Tito_Scwrl_1UP7.pdb: