Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKLFHSTLIVLLFFSFFGVQPIHAKKQFKVPNS---VASISKENTYPNASQDQPMLQPSKLAKELLDHSEVKIENPHLIKMLNESNISGTPLAVGYRATIFLGKWALGYESNETVANWEYKKINTNRADNRGGKETAEMHYAQEQQYRVKGGLTAKVPNAEDVKSMMMQKAMKKTNLPLAFETVIGAGTKRDQIYKVAPKKIGYLHAYAPAVNEKGKVTYGEVYLVLKGNKRKLVVKNVTSQGIGAWIPVQDHVTFGFQLSSLPR 4CRC Chain:A ((72-123)) ----------------SFFGVQEIIIHDQYKMAESGYDIALLKLETTVNYADSQRPISLPSKGERNVI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4CRC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 52 -2910 -55.96 -59.39 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -55.96 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_4CRC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CRC-query.scw PDB file : Tito_Scwrl_4CRC.pdb: