Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSVNLSYPIGEYKPRESISKEQKDKWIQVLEEVPAKLKQAVEVMTDSQ---LDTPYRDGGWTVRQVVHHLA---DSHMNSYIRFKLSLTEETPAIRPYDEKAWSELKDSKTADPSG-SLALLQELHGRWTALLR---TLTDQQFKRGFYHPDTKEIITLENALG---LYVWHSHHHIAHITELSRRMGWS 2YQY Chain:A ((9-160)) -------------------------TWDEALKRLEASRKALLALLREADPAWLSAPLREGAWTPLMVAEHVALVEDSTARVLRRLRRLA----------------------------LSLEEVLALLDRARAFLLEEVAKADPQNPATFPHPFFGEL----NPLGWLRAAAYHEAHHL-------------

General information: TITO was launched using: RESULT: Template: 2YQY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 316 -31681 -100.26 -293.34 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -100.26 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_2YQY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YQY-query.scw PDB file : Tito_Scwrl_2YQY.pdb: