Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVFLIGANGQIGQRLVSLFQDNPDHSI-RAMVRKEEQKASLEAAGAEA-VLANLEGSPEEIAAAAKGCDAIIF--TAGSGGSTGYDKTL-----------LVDLDGAAKAIEAAAIAGIKRFIMVSALQAHNRENWNEALKP--YYVAKHYADKILEASGLTYTIIRPGGLRN-EPGTGTVSAAKDLE-----RGFISRDDVAKTVIASLDEKNTENRAFDLTEGDTPIAEALKKL 1YBM Chain:A ((7-228)) --VLVTGASGRTGQIVYKKLKEGSDKFVAKGLVRSAQGK---EKIGGEADVFIGDITDADSINPAFQGIDALVILTSAVPKMKP------EFIFEDGQYPEQVDWIGQKNQIDAAKVAGVKHIVVVGSMGGTNPDHPLNKLGNGNILVWKRKAEQYLADSGTPYTIIRAGGLLDKEGGVRELLVGKDDELLQTDTKTVPRADVAEVCIQALLFEEAKNKAFDL--------------

General information: TITO was launched using: RESULT: Template: 1YBM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 972 -93153 -95.84 -492.87 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -95.84 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_1YBM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YBM-query.scw PDB file : Tito_Scwrl_1YBM.pdb: