Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIVRIETFPLFHRLEKPYGDANGFKRYRTCYLIRIITESGIDGWGECVD--------------WLPALHVGFTKRIIPFL---LGKQAGSRLSLVRTIQKWHQRAASAVSMALTEIAAKAADCSVCELWGGRYREEIPVYASFQSYSDSPQWISRSVSNVEAQLKKGFEQIKVKIGGTSFKEDVRHINALQHTAGSSITMILDANQSYDAAAAFKWERYFSEWTNIGWLEEPLPFDQPQDYAMLRSRLSVPVAGGENMKGPAQYVPLLSQRCLDIIQPDVMHVNGIDEFRDCLQLARYFGVRASAHAYDGSLSRLYALFAQACLPPWSKMKNDHIEPI-EWDVMENPFTDLVSLQP---SKGMVHIPKGKGIGTEINMEIVNRYKWDGSAY 4HPN Chain:A ((1-374)) MKITAVRTHLLEHRLDTPFESASMRFDRRAHVLVEIECDDGTVGWGECLGPARPNAAVVQAYSGWLIGQDPRQTEKIWAVLYNALRDQGQRGLSLT---------ALSGIDIALWDIKGKHYGASISMLLGGRWRESVRAYATGSFKRDNVDRVSDNASEMAERRAEGFHACKIKIG-FGVEEDLRVIAAVREAIGPDMRLMIDANHGYTVTEAITLGDRAAGF-GIDWFEEPVVPEQLDAYARVRAGQPIPVAGGETWHGRYGMWQALSAGAVDILQPDLCGCGGFSEIQKIATLATLHGVRIVPHVWGTGVQIAAALQFMAAMTP-DPVRVNPIEPIMEFDRTHNPFRQAVLREPLEAVNGVVTIPDGPGLGIEINRDALTEFR------

General information: TITO was launched using: RESULT: Template: 4HPN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2077 -195112 -93.94 -552.72 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -93.94 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.405

(partial model without unconserved sides chains): PDB file : Tito_4HPN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HPN-query.scw PDB file : Tito_Scwrl_4HPN.pdb: