Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHSFAVPRSVEWKETAITILNQQKLPDETEYLELTTKEDVFDAIVTLKVRGAPAIGITAAFGLALAAKDIETDNVTEFRRRLEDIKQYLNSSRPTAINLSWALERLSHSVENAISVNEAKTNLVHEAIQIQVEDEETCRLIGQNALQLFKKGDRIMTICNAGSIATSRYGTALAPFYLAKQKDLGLHIYACETRPVLQGSRLTAWELMQGGIDVTLITDSMAAHTMKEKQISAVIVGADRIAKNGDTANKIGTYGLAILANAFDIPFFVAAPLSTFDTKVKCGADIPIEERDPEEVRQISGVRTAPSNVPVFNPAFDITPHDLISGIITEKGIMTGNYEEEIEQLFKGEKVH 1T5O Chain:A ((4-341)) --------RSIFWDD-GLKLIDQTKLPEKLEVIECRNVEELADAIKKLAVRGAPALEAAGAYGIALAAREREFADVDELKEHLKKAADFLASTRPTAVNLFVGIERALNAALKGESVEEVKELALREAEKLAEEDVERNRKMGEYGAELLEDGDVVLTYCNAGRLATVDWGTALGVVRSAVEQGKEIRVIACETRPLNQGSRLTCWELMEDGIDVTLITDSMVGIVMQKGMVDKVIVGADRIVRDA-VFNKIGTYTVSVVAKHHNIPFYVAAPKATFDWE-RTAKDVVIEERPREELIFCGKRQIAPLNVKVYNPAFDPTPLENVTALITEYGVIYPPYEVNVPKVLKF----

General information: TITO was launched using: RESULT: Template: 1T5O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1958 -201419 -102.87 -595.91 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -102.87 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_1T5O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T5O-query.scw PDB file : Tito_Scwrl_1T5O.pdb: