Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNQTARSSKKERYANLIPMEELHSEKDRLFPFPIYDKLRRESPVRYDPLRDCWDVFKYDDVQFVLKNPKLFSSK-----RGIQTESILTMDPPKHTKLRALVSRAFTPKAVKQLETRIKDVTAFLLQEARQKSTIDIIEDFAGPLPVIIIAEMLGAPIEDRHLIKTYSDVLVAGAKDSSDKAVADMVHNRRDGHAFLS---DYFRDILSKRRAEPKEDLMTMLLQAEIDGEYLTEEQLIGFCILLLVAGNETTTNLIANAVRYLTEDSVVQQQVRQNTDN--VANVIEETLRYYSPVQAIGRVATEDTELGGVFIKKGSSVISWIASANRDEDKFCKPDCFKIDRPSYPHLSFGFGIHFCLGAPLARLEANIALSSLLSMSACIEKAAHDEKLEAIPSPFVFGVKRLPVRITFK 4E2P Chain:A ((35-384)) --------------------------------FWLYGRRPRQM-VEFDEKMNAWNVYGYAEAIEVLGDPGTFSSHMSRLL--FTEGDLLQTDPPDHRELRKLVSHAFTPKVVAELEPRITALTQELLDAVADRDTFDLMTALAYPLPVTVVAELLSIPSADRHLFEGWMTEIVHS-------------------MAPMRKMLEYLREHAAECRRRPRGDLMGKLIEAEVDGRRLTDNHIVNFAKMLLIAGYLTTTMLIGNTV--LCLDSYPEQAARVRADRSLIPGLLEESMRFLSPVAATYRATTRDVEVAGQRLSADQMVMVWFGAANRDARQFAEPELFDMTRGPNPHLGFGRGIHFCLGGPLARMEGRVALDHLL------------------------------------

General information: TITO was launched using: RESULT: Template: 4E2P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1445 -186220 -128.87 -594.95 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -128.87 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_4E2P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E2P-query.scw PDB file : Tito_Scwrl_4E2P.pdb: