Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKAYLTVCSNQQIADRVFQMVLKGELVQGFTTPGQFLHLKVSEAVTPLLRRPISIADVNFEKNEVTIIYRVDGEGTRLLSLKQQGE-LVDVLGPLGNGFPVNEVQPGKTALLVGGGVGVPPLQELSKRLIEKGVNVIHVLGFQSAKDVFYEEECRQYGDTYVATADGSYGETGFVTDVIKR--KKLEFDILLSCGPTPMLK--ALKQEYAHKEVYLSMEERMGCGIGACFACVCHTNESETSYVKVCLDGPVFKAQEVAL 4YLF Chain:A ((9-251)) -------IVKKVKIAEDVFDFWIHSPSVSKEARPGQFVVIRLHEKGE---RIPLTVADTKPEEGLFRMVVKVVGKTTHELSLKKEGDTILDVVGPLGNP---SEIENYGNVLLVGGGVGIATLYPIAKALKEAGNNITTVLGARTKDYLIMVDEFKEISDVLLVTDDGSAGMKGVVTDAMDKLFRERKFDICWAVGPTIMMKFCTLKAREFGVPIWVSLNPIMVDGTGMCGACRV-TVSGQIKF--ACVDGPEFRGEEV--

General information: TITO was launched using: RESULT: Template: 4YLF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1355 -141504 -104.43 -594.55 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -104.43 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_4YLF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YLF-query.scw PDB file : Tito_Scwrl_4YLF.pdb: