Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKWIVLFLVLIAAAISIFVYVSTGSEKPFYNDINLTQYQKEVDSKKPKFIYVYETSCPPCQEIKPELNEVIK--KEKLKVQALNIEEKENYNTEFLDKYNLNKTPTILYYKDGKEKDRLEGYRSASQIEKFFDKNGDR 4POB Chain:A ((27-114)) ------------------------------------------VSSNKPVLVDFWATWCGPCKMVAPVLEEIAKDHGEALTIAKLDV----DANPETARAFQVTSIPTLILFQNGEATKRIVGAKSKSALLRELD-----

General information: TITO was launched using: RESULT: Template: 4POB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 325 -12927 -39.77 -150.31 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -39.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.781

(partial model without unconserved sides chains): PDB file : Tito_4POB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4POB-query.scw PDB file : Tito_Scwrl_4POB.pdb: