Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIVESTTFPRTKQSITEDLKALGLKKGMTVLVHSSLSSIGWVNGGAVAVIQALIDVVTEEGTIVMPSQSVELSDPKEWGNPPVPEEWWDIIRESMPAYNSNYTPTTRGMGQIVELFRSYPEVKRSNHPNYSFVAWGKHKNKILNQHPLEFGLGEQSPLGKLYIRESYVLLLGADFDSSTCFHLAEYRIPYQKIINRGAPIIVEGKRVWKEYKELEFREELFQEVGQAFEAEHNMKVGKVGSANCRLFSLTEAVDFAEKWFINNDSKNIKK 3E4F Chain:A ((4-261)) ---IVASTQLPNTIKTITNDLRKLGLKKGMTVIVHSSLSSIGWISGGAVAVVEALMEVITEEGTIIMPTQSSDLSDPKHWSRPPVPEEWWQIIRDNVPAFEPHITPT-RAMGKVVECFRTYPNVVRSNHPLGSFAAWGRHAEEITVNQSLSMSLGEESPLRKIYDLDGYILLIGVGYDSNTSVHLSEVRSGACELIKVGAPIIENGERVWKEFVDMDYDSDKFVEIGVEFEQKGTVTMGKIGNAKCRLMKQRDIVDFGTEWF----------

General information: TITO was launched using: RESULT: Template: 3E4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1455 -156279 -107.41 -605.73 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -107.41 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.547

(partial model without unconserved sides chains): PDB file : Tito_3E4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E4F-query.scw PDB file : Tito_Scwrl_3E4F.pdb: