Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLADKVKLSAKEILEKEFKTGVRGYKQEDVDKFLDMIIKDYETFHQEIEELQQENLQLKKQLEEASKKQPVQSNTTNFDILKRLSNLEKHVFGSKLYD 2WUK Chain:A ((2-47)) ------PLTPNDIHNKTFTKSARGYDEDEVNEFLAQVRKDYEIVLRKKTELE----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2WUK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 59 -6396 -108.40 -139.03 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -108.40 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.740

(partial model without unconserved sides chains): PDB file : Tito_2WUK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WUK-query.scw PDB file : Tito_Scwrl_2WUK.pdb: