Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENKIIYFLCTGNSCRSQMAEGWAKQYLGDEWKVYSAGIEAHGLNPNAVKAMKEVGIDISNQTSDIIDSDILNNADLVVTLCGDAADKCPMTPPHVKREHWGFDDPARAQGTEEEKWAFFQRVRDEIGNRLKEFAETGK 2IPA Chain:B ((1-139)) MENKIIYFLSTGNSARSQMAEGWAKQYLGDEWKVYSAGIEAHGLNPNAVKAMKEVGIDISNQTSDIIDSDILNNADLVVTLSGDAADKCPMTPPHVKREHWGFDDPARAQGTEEEKWAFFQRVRDEIGNRLKEFAETGK

General information: TITO was launched using: RESULT: Template: 2IPA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 722 -33987 -47.07 -244.51 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 1.00 3D Compatibility (PKB) : -47.07 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_2IPA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IPA-query.scw PDB file : Tito_Scwrl_2IPA.pdb: