Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGIGARDLGIDLGTANTLVFVKGKGIVVREPSVVALQT--DTKSIVAVGNDAKNMIGRTPGNVVALRPMKDGVIADYETTATMMKYYINQAIKNKGMFARKPYVMVCVPSGITAVEERAVIDATRQAGARDAYPIEEPFAAAIGANLPVWEPTGSMVVDIGGGTTEVAIISLGGIVTSQSIRVAGDEMDDAIINYIRKTYNLMIGDRTAEAIKMEIGSAEAPEESDNM--EIRGRDLLTGLPKTIEITGKEISNALRDTVSTIVEAVKSTLEKTPPELAADIMDRGIVLTGGGALLRNLDKVISEETKMPVLIAEDPLDCVAIGTGKALEHIHLFKGKTR 4CZH Chain:A ((3-327)) -----SNDIAIDLGTANTLIYQKGKGIVLNEPSVVALRNVGGRKVVHAVGIEAKQMLGRTPGHMEAIRPMRDGVIADFEVAEEMIKYFIRKVHNRKG--SGNPKVIVCVPSGATAVERRAINDSCLNAGARRVGLIDEPMAAAIGAGLPIHEPTGSMVVDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRHHNLLIGETTAERIKKEIGTARAP----GLSIDVKGRDLMQGVPREVRISEKQAADALAEPVGQIVEAVKVALEATPPELASDIADKGIMLTGGGALLRGLDAEIRDHTGLPVTVADDPLSCVALGCGKVLEH---------

General information: TITO was launched using: RESULT: Template: 4CZH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1859 6204 3.34 19.57 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : 3.34 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_4CZH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZH-query.scw PDB file : Tito_Scwrl_4CZH.pdb: