Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSKFEASIDNLKEIEMNAYAYELIREIVLPDMLGQDYSSMMYWAGKHLARKFPLESWEEFPAFFEEAGWGTLTNVSAK----KQELEFELEGPIISNRLKHQKEPCFQLEAGFIAEQIQLMNDQIAESYEQVKK--RADKVVLTVKWDMKDPV 2OSO Chain:A ((22-157)) --------IKESKKIPMPYFGLFALVIFDKVK--GSE--TSLYEIGEEFGKMLSPKNIEELKKIFKLMNFGDLEIDENKILLKNPPYKIKLSNPPY--QWVSKEEPIHDFIAGILAGCLEEIFYYYFVVNEVECVSQGKDKCVFEVKE------

General information: TITO was launched using: RESULT: Template: 2OSO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 469 -21784 -46.45 -170.19 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -46.45 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_2OSO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OSO-query.scw PDB file : Tito_Scwrl_2OSO.pdb: