Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKITFIGAGSTIFAKNVLGDCLLTEALNGFEFALYDIDPKRLQESQLMLENLRDRYNPSVAINSYDDRKLALQNAGYVINAIQVGGYKPSTVIDFEIPKRYGLRQTIADTVGIGGIFRSLRTIPVLFDIAKDMEEMCPDAWFLNYTNPMATLTGAMLRY-TNIKTIGLCHSVQVCTKDLFKALGME-HDGIEERIAGINHMAWLLEVK-KDGTDLYPEIKRRAKEKQKTKHHDMVRFELMDKFGYYV-TESSEHNAEYHPYFIK-RNY----PELISELQIPLDEYPRRCVKQIENWEKMRDDIVNNKN---------LTHERSKE--------YGSRIIE-----AMETNEPFTFGGNVLNTGLITNLPSKAVVEVTCVADRKKITPCFAGELPEQLAALNRTNINTQLMTIEAAVTRKKEAVYQAAMLDPHTSAELSMKDIISMCDDLFAAHGDWLPEYK 1U8X Chain:X ((31-463)) ---IVIAGGGSTFTPGIVLMLLDHLEEFPIRKLKLYDNDKERQDRIAGACDVFIREKAPDIEFAATTDPEEAFTDVDFVMAHIRVGKYA-MRALDEQIPLKYGV--VGQETCGPGGIAYGMRSIGGVLEILDYMEKYSPDAWMLNYSNPAAIVAEATRRLRPNSKILNICDMPVGIEDRMAQILGLSSRKEMKVRYYGLNHFGWWTSIQDQEGNDLMPKLK-----------------EHVSQYGYIPKT-------SWNDTFAKARDVQAADPDTLPNTYLQYYLFPDDMVKKSNPNHTRANEVMEGREAFIFSQCDMITREQSSENSEIKIDDHASYIVDLARAIAYNTGERMLL--IVENNGAIANFDPTAMVEVPCIVGSNGPEPITVGTIPQFQKGLMEQQVSVEKLTVEAWAEKSFQKLWQALILSKTVPNARVARLIL---EDLVEANKDFWPE--

General information: TITO was launched using: RESULT: Template: 1U8X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 2180 -76244 -34.97 -193.02 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain X : 0.75 3D Compatibility (PKB) : -34.97 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_1U8X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U8X-query.scw PDB file : Tito_Scwrl_1U8X.pdb: