Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLNDILEFNKTFTEQREYEKYQTSKFPDKKMAILSCMDTRLVELLPHAMNLRNGDVKIIKSAGALVTHPFGSIMRSILVAVYELNADEVCVIGHHDCGMSKISSKSMLEKIKARGI-----------PEERIETIKYSGVDFDQWFKSFDSVEASVKD-----SVDVIKHHPLFPENVPVHGLVIDPKTGKLDLIVNGYNN 3TEO Chain:A ((27-182)) -----------------------------NRRLWVLTCMDERV--HIEQSLGIQPDDAHIYRNAGGIVT---DDAIRSASLTTNFFGTKEIIVVTHTDCGMLRFTGEEVAKYFISKGIKPTEVQLDPLLPAFRIS----SEEDFIKWFKFYEDLGVKSPDEMALKGVEILRNHPLIPKDVRITGYVYEVETHRL---------

General information: TITO was launched using: RESULT: Template: 3TEO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 556 -59249 -106.56 -423.20 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -106.56 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_3TEO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TEO-query.scw PDB file : Tito_Scwrl_3TEO.pdb: