Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLSVGDEVQVKVLAVDEEKGKISLSIRATQAAPE-KKESKPRKPKAAQVSEEAST---PQGFNTLKDKLEEWIEMSNRKDLIKK 4NNI Chain:A ((16-128)) ------IGQIVPGKVTKLVPFGAFVRVEEGIEGLVHISELAERHVEVPDQVVAVGDDAMVKVIDIDLERRRISLSLK--QANEDYTEEFDPAKYGMADSYDEQGNYIFPEGFDA---ETNEWLE----------

General information: TITO was launched using: RESULT: Template: 4NNI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 423 -34405 -81.33 -315.64 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -81.33 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_4NNI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NNI-query.scw PDB file : Tito_Scwrl_4NNI.pdb: