Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTICVFAGSNPGGNEAYKRKAAELGVYMAEQGIGLVYGGSRVGLMGTIADAIME-NGGTAI-GVMPSGLFSGE------VVHQNL----TELI-----EVNGMHERKAKMSE------LADGFISMPGGFGTYEELFEVLCWAQIGIHQKPIGLYNVNGYFEPMMKMVKYSIQEGFSNESHLKLIHSSSRPDELIEQMQNYSYPILEKKWTEI 5AJT Chain:A ((62-260)) ---ICVFCGSSGGASPAHMEAARQLGRVMAENNIDLVYGGGTVGLMGEVARTVCSINGPESVHGIIPEALVRYERDGTYQTVKDNKQVVPTETVYGRTTVVKDMHTRKKMMAEEVISGGPGSGFIGLSGGYGTMEEVFEVITWNQLGIHTKGICLLNVEGYWDGILQWINMAAAQGFVQPGNETIVVSAGDAEGAVRALREY------------

General information: TITO was launched using: RESULT: Template: 5AJT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 833 -30180 -36.23 -171.47 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -36.23 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_5AJT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AJT-query.scw PDB file : Tito_Scwrl_5AJT.pdb: