Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKRIIPCLDVKEGRVVKGIQFLELKDAGDPVELAEVYDREGADELVFLDISASHEGRKTMVDVVEQVAAKLAIPFTVGGGINQLSDMKTILRAGADKVSVNTAAVLRPELITEGAEFFGSQCIVLAIDAKYDKESDTYKVYTHGGRKKTDWEVTAWAKEGVKRGAGEILLTSMDSDGEKKGFDHTLTKLVSEAVPVPVIASGGAGNAQHMLEAFTKGEADAALAASIFHYKETSIKEVKSYLKEYGVNVR 2A0N Chain:A ((13-261)) MLAKRIIACLDVKDGRVVKGTNFENLRDSGDPVELGKFYSEIGIDELVFLDITASVEKRKTMLELVEKVAEQIDIPFTVGGGIHDFETASELILRGADKVSINTAAVENPSLITQIAQTFGSQAVVVAIDAK--RVDGEFMVFTYSGKKNTGILLRDWVVEVEKRGAGEILLTSIDRDGTKSGYDTEMIRFVRPLTTLPIIASGGAGKMEHFLEA-FLAGADAALAASVFHFREIDVRELKEYLKKHGVNVR

General information: TITO was launched using: RESULT: Template: 2A0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1433 -152613 -106.50 -612.90 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -106.50 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.605

(partial model without unconserved sides chains): PDB file : Tito_2A0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A0N-query.scw PDB file : Tito_Scwrl_2A0N.pdb: