Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFYRVEQMPGFIKTEMQKIQKAVQPFMKKTVIYRFLAIPLAAFSLFNL--------------AAFLFHA---SADRESLISAGIFALLAALGLAFFKEAGYQHKQIQKTVHIYMLNRIKKSEILSEERKSSYARQIKEEPFAMRSFVEFLTEEDRRKKMY 3EJE Chain:B ((105-229)) ------ESYQPYIIETVHHLLDQVQGKKKMEVISDF-AFPLASFVIANIIGVPEEDREQLKEWAASLIQTIDFTRSRKALTEGNIMAVQA---MAYFKELIQKRKRHPQQDMISMLLK-----KLTEEEAAS-----------------------------

General information: TITO was launched using: RESULT: Template: 3EJE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 182 -14128 -77.63 -141.28 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -77.63 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.135

(partial model without unconserved sides chains): PDB file : Tito_3EJE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EJE-query.scw PDB file : Tito_Scwrl_3EJE.pdb: