Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANEFRVCDDCQA-TNIKTLLPRLKEVDPDA--KVEVGCQSYCGPGRKKSFAFVNNRPLSA-------PTEDELIDKVKKKIKK 2OKA Chain:A ((7-83)) -EIVITYCTQCQWLLRAAWLAQELLSTFADDLGKVCLE-PG----TGGVFRITCDGVQVWERKADGGFPEAKALKQRVRDRID-

General information: TITO was launched using: RESULT: Template: 2OKA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 230 1143 4.97 17.06 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 4.97 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_2OKA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OKA-query.scw PDB file : Tito_Scwrl_2OKA.pdb: