Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MINSIQWEYIFNTKLAIESFPYVIK-----GIGYTLLISFVSM-FAGTVIGLFISLARMSKLALLRWPAKLYISFMRGVPILVILFILYFGFPYI----GIEFSAVTAALI-----------GFSLNSAAYIAEINRSAISSVEKGQWEAASSLGLSYWQTMRGIILPQSIRIALPPLANVLLDLIKASSLAAMITVPELLQHAKIIGGREFDYMTMYILTALIYWAICSIAAVFQNILE-KKYAHYV------------------------------------------------------------------------------------------- 4D08 Chain:A ((4-342)) DDEYTKLLHDGIQPVAAIDSNFA--SFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCN----IFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLCLL-MTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLD

General information: TITO was launched using: RESULT: Template: 4D08.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 827 -165150 -199.70 -754.11 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -199.70 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.253

(partial model without unconserved sides chains): PDB file : Tito_4D08.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D08-query.scw PDB file : Tito_Scwrl_4D08.pdb: